Molecular cloning of cat interleukin-4
نویسندگان
چکیده
منابع مشابه
Cloning of a full length cDNA encoding canine Interleukin-4.
Primer designed on the basis of the conserved transcription start point (tsp) region of human and bovine gene transcripts, encoding the Interleukin-4 (IL-4), and subsequently the gene specific primer and an adaptor primer pair, was successfully used, to generate the full-length complementary DNA (cDNA) sequence coding for canine IL-4 (cIL-4), from pokeweed mitogen stimulated canine peripheral b...
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Murine interleukin-5 (IL-5) is known to play an essential role in Ig production of B cells and proliferation and differentiation of eosinophils. Here, we have isolated cDNA clones encoding a murine IL-5 receptor by expression screening of a library prepared from a murine IL-5 dependent early B cell line. A cDNA library was expressed in COS7 cells and screened by panning with the use of anti-IL-...
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The present study describes the cloning and functional characterization of avian interleukin (IL)-19, a cytokine that, in mammals, alters the balance of Th1 and Th2 cells in favor of the Th2 phenotype. The full-length avian IL-19 gene, located on chromosome 26, was amplified from LPS-stimulated chicken monocytes, and cloned into both prokaryotic (pET28a) and eukaryotic (pcDNA3.1) expression vec...
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Interleukin-2 (IL-2 or T cell growth factor) is a 14,500 daltons glycoprotein critically involved in the development of a normal human immune response [1, 2J. Recently, cDNA for this lymphokine has been isolated and expressed in both prokaryotic and eukaryotic cells [3-5J. Further, the human IL-2 gene has been cloned, sequenced [6], and localized to chromosome 4. As with other polypeptide hormo...
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We reported molecular cloning of B cell stimulatory factor-2 (BSF-2)/interleukin 6 (IL-6) in 1986 (1), the same year that IL-4 and IL-5 were cloned (2, 3). Prior to the publication of our article (1), it was known that antigenic stimulation induced growth and differentiation of B lymphocytes into antibody forming plasma cells with the help of T lymphocytes and this function of T lymphocytes cou...
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ژورنال
عنوان ژورنال: Immunogenetics
سال: 1995
ISSN: 0093-7711,1432-1211
DOI: 10.1007/bf00179411